The Toxicological Laboratory has been working on the animal use reduction and, in 2019, .opened its In vitro Toxicological Laboratory. Comparative studies has shown a similarity between results of in vivo and in vitro studies, making this implementation safe and viable.

Below, a relation of In vitro studies following OECD normative:

• Bovine corneal opacity and permeability test (BCOP) (OECD 437);
• Isolated chicken eye test – ICE (OECD 438);
• Short term exposure in vitro for eye risk evaluation – STE (OECD 491);
• In vitro skin corrosion test. Assay with reconstructed human epidermis (RHE) (OECD 431);
• In vitro skin irritation test. Assay with reconstructed human epidermis (RHE) (OECD 439);
• Local lymph node assay: BRDU-ELISA (OECD 442B);

Genotoxicity and mutagenicity studies

Genotoxicity and mutagenicity studies are performed to predict genotoxic and mutagenic potential of a specific substance, through gene mutation, chromosomal damage or DNA lesion evaluation, which are important to adverse outcome induction in human health, such as cancer.

• Micronucleous test in vitro – MNVit (OECD 487)
• Reversal gene mutation test (Ames Test) (OECD 471)
• In vitro mammalian cell gene mutation assay using cells from mouse lymphoma – MLA (OECD 490)

The GLP 0021 scope can be consulted at INMETRO webpag